ARPE-19 cell line

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location： Home > Products > Wild-type Cell Lines > Human Retinal Pigment Epithelial Cell Line(ARPE-19)

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Human Retinal Pigment Epithelial Cell Line(ARPE-19) Order

ARPE-19 cell line
Tissue
Human Retinal Pigment Epithelial Cell Line
Catalog#
YC-C033
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Datasheet
ARPE-19 cell line
Order

Detailed Product Information

Product category
Wild-type Cell Lines
Cell Info
ARPE-19 is a spontaneously arising retinal pigment epithelia (RPE) cell line derived in 1986 by Amy Aotaki-Keen from the normal eyes of a 19-year-old male who died from head trauma in a motor vehicle accident.The line was established in a 1:1 mixture of Dulbecco's modified Eagles medium and Ham's F12 medium with HEPES buffer containing 20% fetal bovine serum, 56 mM final concentration sodium bicarbonate and 2 mM L-glutamine and incubated at 37C in 10% CO2. The cells were subjected to selective trypsinization for the first four passages to remove superficial cells before passaging the cuboidal basal layer. By passage 5, the cultures appeared to be rapidly growing RPE cells, which would form cobblestone monolayers, which pigmented after several months in culture.
Morphology
Endothelial cells, adherent
Passage ratio
1:3~1:5
Mycoplasma test
negative
Culture method
90% DMEM/F12+10% FBS
Accession
CVCL_0145(Cellosaurus)
ExPASy Cellosaurus Database
Is a valuable online resource that compiles detailed information on over 100,000 cell lines from reputable cell banks such as ATCC, ECACC, and DSMZ. The database provides information including descriptions, origins, and characteristics of the cell lines, with broad reference value and scientific reliability. Additionally, the database provides information on cell line authentication, STR profiles, and potential contamination risks, helping researchers ensure the trustworthiness of the cell lines used in their experiments.
STR Profile
Loci STR Info (Sample Cell)
Sample Cell Line:ARPE-19		 STR Info (Cell bank)
Cell Line:ARPE-19
Allele1Allele2Allele3Allele1Allele2Allele3
D5S818 13131313
D13S317 11121112
D7S820 911911
D16S539 911911
VWA 16191619
TH01 69.369.3
AMEL XYXY
TPOX 911911
CSF1PO 11111111
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases
Cultured cell morphology
ARPE-19 cell line Cultured cell morphology
ARPE-19 cell line Cultured cell morphology

Product Advantage

  • High-quality cells: The cells in our cell bank have low passages, high activity and good cell condition
    01
  • STR Authentication: All cells of human and murine origin in our cell bank have available STR Authentication reports, guaranteeing accurate cell identity.
    02
  • Applicable for gene-editing: The cell lines in our cell bank have been successfully used in gene-editing cell line generation, applicable for all kinds of gene-editing experiments.
    03
  • Free technical support: Ubigene, with over 12-year gene-editing experience, will provide the professional technical support of gene-editing technology for free.
    04

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